This is done by determining the rate at which the intensity of the scattered light fluctuates when detected using a suitable optical arrangement. Dynamic light scattering (DLS) is an optical measurement technique for the character-ization of disperse systems. In dynamic light scattering, the speed at which the particles diffuse due to Brownian motion is measured. In this third white paper of the series, we cover the basic types of DLS deconvolution algorithms used to extract the intensity weighted particle size distribution from the measured correlogram. decay rate (s 1) dynamic viscosity (Pa s). In this four-part white paper series, common issues and questions surrounding the principles, measurements and analysis of DLS data are discussed in order to help minimize the time required for and complexity of acquiring and interpreting DLS data that is critical throughout the development process. The calculated diameters of latex spheres in 75 (v/v) glycerol solution. Thus, it is equivalent to reducing the required high frame rate. So, by dynamic light scattering one has determined the intensity weighted. Numerical simulation in Table 3 shows that the decay rate in water reduced to the same level of that in 75 (v/v) glycerol solution by decreasing the scattering angle from 45° to 10°. With that said, the challenge that remains with the application of DLS to protein therapeutic formulations is centered around data interpretation. Recall that the decay constant is proportional to the diffusion coefficient. The popularity of DLS within the biopharmaceutical industry is a consequence of its wide working size and extended sample concentration ranges, as well as its low volume requirements. Dynamic light scattering (DLS) is an analytical technique used to measure the particle size distribution of protein formulations across the oligomer and sub-micron size ranges of approximately 1 nm to 1 µm. Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. D ynamic L ight S cattering (DLS), sometimes referred to as P hoton C orrelation S pectroscopy or Q uasi-Elastic L ight S cattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid.
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